基于金属——有机骨架材料的C反应蛋白传感器研制

李志鹏; 刘仪; 李云蕊; 卢明月; 杜雪婷; 杨云慧

doi:10.13873/j.1000-9787(2017)11-0078-05

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基于金属——有机骨架材料的C反应蛋白传感器研制

设计与制造 | 更新时间：2025-09-11

- 基于金属——有机骨架材料的C反应蛋白传感器研制
- Research and fabrication of CRP immunosensor based on MOFs materials
- 传感器与微系统 2017年36卷第11期页码：78-82
- 作者机构：
  
  1. 云南师范大学化学化工学院
  2. 昭通市疾病预防控制中心
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目（21465026,21165023）
- DOI：10.13873/j.1000-9787(2017)11-0078-05
  中图分类号： R446.6;TP212
- 纸质出版：2017
- 稿件说明：
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[1]李志鹏,刘仪,李云蕊,卢明月,杜雪婷,杨云慧.基于金属——有机骨架材料的C反应蛋白传感器研制[J].传感器与微系统,2017,36(11):78-82.

LI Zhi-peng, LIU Yi, LI Yun-rui, et al. Research and fabrication of CRP immunosensor based on MOFs materials[J]. 2017, 36(11): 78-82.
[1]李志鹏,刘仪,李云蕊,卢明月,杜雪婷,杨云慧.基于金属——有机骨架材料的C反应蛋白传感器研制[J].传感器与微系统,2017,36(11):78-82. DOI： 10.13873/j.1000-9787(2017)11-0078-05.

LI Zhi-peng, LIU Yi, LI Yun-rui, et al. Research and fabrication of CRP immunosensor based on MOFs materials[J]. 2017, 36(11): 78-82. DOI： 10.13873/j.1000-9787(2017)11-0078-05.

摘要

在常温下合成了Au纳米颗粒负载的超纳米结构金属有机骨架化合物（Au NPs/SNHKUST—1）

并以其为标记材料标记C反应蛋白抗体。同时合成了中空状石墨烯纳米材料和Au纳米颗粒作为固定基质

制备了夹心型的C反应蛋白免疫传感器。通过检测标记物对H

还原反应的催化电流

实现了对C反应蛋白的定量测定。传感器在C反应蛋白浓度为0.2

00 ng/m L的范围内线性良好的

线性相关系数R

=0.994 7

检测下限为0.05 ng/m L

为C反应蛋白测定提供了一种新的简单快速的检测方法。

Abstract

Au nanoparticles-loaded metal organic framework compounds with ultranano-structured（ Au/SNHKUST-1） is synthesized at room temperature and use it as a tag to label anti C-creative protein（ CRP）. Hollow graphene nanomaterials and Au nanoparticles are also synthesized and utilized as immobilized matrix. The quantitative determination of CRP can be achieved by detecting the catalytic current of label to the reduction of H

. The response current has a good linear relationship with CRP concentration at range of 0.2

200 ng/m L with linear correlation coefficient of 0.9947 and the lowest detection limit is 0.05 ng/m L. This immunosensor of CRP provides a new simple and rapid detection me

thod for CRP.

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references

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