CHENG Zu-le, WANG Kun, MAO Hong-ju, et al. Emulsion digital PCR quantitative method based on microbeads and micropillar array chip[J]. 2017, 36(5): 16-18.
CHENG Zu-le, WANG Kun, MAO Hong-ju, et al. Emulsion digital PCR quantitative method based on microbeads and micropillar array chip[J]. 2017, 36(5): 16-18. DOI: 10.13873/j.1000-9787(2017)05-0016-03.
BEAMing is an emulsion digital polymerase chain reaction( PCR) technology with high sensitivity.However
the available beads detecting method is complex
which limits its ’ applications. Through counting the streptavidin modified polystyrene latex microspheres which have fully captured the target magnetic beads
the number of target magnetic beads can be calculated easily. The microsphere trapping mechanism is based on the difference of microsphere diameter and micropillars intervals. The fabrication of this chip is simple and low-cost.This chip-and-beads-based method reduces difficulty of magnetic beads counting in BEAMing. Feasibility of this method is demonstrated by detecting a DNA sequence in different concentrations.
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