1. 山东省菏泽生态环境监测中心
2. 菏泽学院化学化工学院
纸质出版:2025
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孙章华, 陈美凤, 马心英, 等. 金/鸟氨酸传感器对奥司他韦的检测及与DNA作用[J]. 传感器与微系统, 2025,(4):21-25+30.
孙章华, 陈美凤, 马心英, 等. 金/鸟氨酸传感器对奥司他韦的检测及与DNA作用[J]. 传感器与微系统, 2025,(4):21-25+30. DOI: 10.13873/J.1000-9787(2025)04-0021-05.
磷酸奥司他韦(OP)是一种神经氨酸酶抑制剂,临床上用于治疗A和B型流感病毒。实验通过在最佳聚合条件下,在玻碳电极(GCE)上电化学聚合纳米金(AuNPs)/聚L—鸟氨酸(PL-Orn)
制备了一种用于测定OP的高灵敏度复合传感器(AuNPs/PL-Orn/GCE)。采用循环伏安法(CV)和扫描电镜(SEM)对该电极进行了表征:PL-Orn在GCE表面形成了一层均匀的膜,AuNPs在PL-Orn均匀地分散,呈针簇状。在最佳测定条件下,该修饰电极可用于OP的测定,线性范围为8.00×10
-8
~2.00×10
-5
mol/L
相关系数R为0.994 1
检出限为4.00×10
-8
mol/L。把该电极用于OP胶囊样品的测定,结果满意。利用电化学方法和紫外可见光谱研究了OP与DNA的相互作用,结果表明:OP与DNA发生了增色效应。
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