1. 广东工业大学材料与能源学院
2. 广东工业大学机电工程学院
纸质出版:2025
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叶锦华, 郭琦伟, 甄铁山, 等. 微型PCR温控系统构建及性能分析[J]. 传感器与微系统, 2025,(3):89-92+97.
叶锦华, 郭琦伟, 甄铁山, 等. 微型PCR温控系统构建及性能分析[J]. 传感器与微系统, 2025,(3):89-92+97. DOI: 10.13873/J.1000-9787(2025)03-0089-04.
针对传统的聚合酶链式反应(PCR)系统体积庞大、反应腔内的温度波动大、扩增效率低和循环耗时长等问题,构建了一种静止式微型PCR系统,并结合实验测试和数值模拟分析对控温模块进行性能优化及分析。系统采用半导体制冷片作为控温元件,铝块作为试管装载导热块,热管结合风冷作为散热元件,采用PID进行控温。实验结果表明:通过分段PID可有效降低升降温过程的温度过充,构建的系统可实现PCR各阶段温度的快速切换,试剂最高升温及降温速率可达5.7℃/s和5.1℃/s
整体反应过程的温度波动在±0.4℃内,系统在70 min完成PCR扩增,相对参照的PCR分析仪,时间可缩短21.3%。
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