上海大学微电子学院
纸质出版:2025
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梁权伟, 关一民. 基于微流控芯片和细胞检测的单细胞分离方法[J]. 传感器与微系统, 2025,(3):76-79+83.
梁权伟, 关一民. 基于微流控芯片和细胞检测的单细胞分离方法[J]. 传感器与微系统, 2025,(3):76-79+83. DOI: 10.13873/J.1000-9787(2025)03-0076-04.
为了实现高通量、高准确性和高克隆活性的单细胞分离。提出了一种基于微流控芯片和单细胞检测的新型单细胞分离方法。并对该方法在检测仓鼠卵巢细胞亚株(CHO-K1)细胞的准确性以及分离后的单细胞克隆活性进行了研究。首先,以CMOS-MEMS微流控芯片为核心,设计了识别640个喷嘴轮廓并定位喷嘴号的图像算法,将单细胞图像送入训练好的卷积神经网络(CNN)中,检测轮廓清晰、形状合适的单个细胞。然后,微流控芯片上的加热电阻通过解码数据通电,利用热发泡喷墨打印原理将单个细胞瞬间喷射到指定位点,将识别到的单细胞连续打印。实验结果表明:CHO-K1单细胞检测平均精度可以达到94.03%
单克隆存活率可以达到87.18%。与有限稀释法(单细胞率提高70.42%
单克隆率提高66.68%)相比,该方法的性能显著提高。
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单子龙,祝明杰,宁伟,吴炫烨.基于微流控与喷墨打印技术的微液滴形成芯片[J].传感器与微系统,2023(01).
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