1. 中国科学技术大学精密机械与精密仪器系
2. 中国科学技术大学安徽省高等学校精密科学仪器重点实验室
纸质出版:2025
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关冠, 赵钢. 微流控细胞变形阻抗检测及其在细胞分选中的应用[J]. 传感器与微系统, 2025,(1):165-168.
关冠, 赵钢. 微流控细胞变形阻抗检测及其在细胞分选中的应用[J]. 传感器与微系统, 2025,(1):165-168. DOI: 10.13873/J.1000-9787(2025)01-0165-04.
基于细胞变形的单细胞检测与分选方法在生物学及医学领域中广泛应用。然而,当前的许多研究以机械挤压的方式使细胞变形,这导致了低通量和通道堵塞等问题。以鞘流剪切的方式使细胞变形,通过阻抗检测得到与细胞机械性能相关的参数,提高了检测准确度,并在此基础上实现了对Hela细胞的高纯度分选。这种方法可用于表征细胞的介电特性、机械性能及无标记地分选细胞,在单细胞的检测与分选中有着广阔的应用前景。
郑力升,王一鸣,李保庆,褚家如.微流控电阻抗检测及其在颗粒分选中的应用[J].传感器与微系统,2022(05).
徐铁,涂亚庆,牟泽龙,陈鹏.复阻抗检测技术研究现状分析及展望[J].传感器与微系统,2019(06).
Xue Li,Guo Ruya,Jin Nana,Wang Siyuan,Duan Hong,Qi Wuzhen,Wang Lei,Zheng Yuanjin,Li Yanbin,Lin Jianhan.Rapid and automatic Salmonella typhimurium detection integrating continuous-flow magnetic separation and dynamic impedance measurement[J].Food Control,2023.
Bruijn Douwe S.,Van de Waal Dedmer B.,Helmsing Nico R.,Olthuis Wouter,Berg Albert.Microfluidic Impedance Cytometry for Single‐Cell Particulate Inorganic Carbon:Particulate Organic Carbon Measurements of Calcifying Algae[J].Global Challenges,2022.
Feng Yongxiang,Chai Huichao,He Weihua,Liang Fei,Cheng Zhen,Wang Wenhui.Impedance-Enabled Camera-Free Intrinsic Mechanical Cytometry.[J].Small methods,2022.
Tang Dezhi,Jiang Lin,Tang Wenlai,Xiang Nan,Ni Zhonghua.Cost-effective portable microfluidic impedance cytometer for broadband impedance cell analysis based on viscoelastic focusing[J].Talanta,2022.
Petchakup Chayakorn,Yang Haoning,Gong Lingyan,He Linwei,Tay Hui Min,Dalan Rinkoo,Chung Aram J,Li King Ho Holden,Hou Han Wei.Microfluidic Impedance-Deformability Cytometry for Label-Free Single Neutrophil Mechanophenotyping.[J].Small (Weinheim an der Bergstrasse, Germany),2022.
Liang Minhui,Yang Dahou,Zhou Yinning,Li Peixian,Zhong Jianwei,Ai Ye.Single-Cell Stretching in Viscoelastic Fluids with Electronically Triggered Imaging for Cellular Mechanical Phenotyping.[J].Analytical chemistry,2021.
Li Peixian,Ai Ye.Label-Free Multivariate Biophysical Phenotyping-Activated Acoustic Sorting at the Single-Cell Level.[J].Analytical chemistry,2021.
Honrado Carlos,Bisegna Paolo,Swami Nathan S,Caselli Federica.Single-cell microfluidic impedance cytometry: from raw signals to cell phenotypes using data analytics.[J].Lab on a chip,2020.
Pillar of Engineering Product Development, Singapore University of Technology and Design, Singapore 487372, Singapore.,BioSystems and Micromechanics IRG (BioSyM), Singapore-MIT Alliance for Research and Technology (SMART) Centre, Singapore 138602, Singapore.,Pillar of Engineering Product Development, Singapore University of Technology and Design, Singapore 487372, Singapore.,BioSystems and Micromechanics IRG (BioSyM), Singapore-MIT Alliance for Research and Technology (SMART) Centre, Singapore 138602, Singapore,Department of Electrical Engineering and Computer Science, and Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.,Pillar of Engineering Product Development, Singapore University of Technology and Design, Singapore 487372, Singapore. Electronic address: aiye@sutd.edu.sg..Biophysical phenotyping of single cells using a differential multiconstriction microfluidic device with self-aligned 3D electrodes[J].Biosensors and Bioelectronics,2019.
Fern J. Armistead,Julia Gala De Pablo,Hermes Gadêlha,Sally A. Peyman,Stephen D. Evans.Cells Under Stress: An Inertial-Shear Microfluidic Determination of Cell Behavior[J].Biophysical Journal,2019.
Biotechnology Center, Technische Universität Dresden, Dresden, Germany,Biotechnology Center, Technische Universität Dresden, Dresden, Germany,Biotechnology Center, Technische Universität Dresden, Dresden, Germany,Biotechnology Center, Technische Universität Dresden, Dresden, Germany,Biotechnology Center, Technische Universität Dresden, Dresden, Germany,Biotechnology Center, Technische Universität Dresden, Dresden, Germany,Biotechnology Center, Technische Universität Dresden, Dresden, Germany,Cavendish Laboratory, University of Cambridge, Cambridge, UK,Biotechnology Center, Technische Universität Dresden, Dresden, Germany,Department of Physics, Creighton University, Omaha, Nebraska, USA,Biotechnology Center, Technische Universität Dresden, Dresden, Germany,Medizinische Klinik 1, Universitätsklinikum Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany,Klinik und Poliklinik für Kinder und Jugendmedizin, Universitätsklinikum Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany,Cavendish Laboratory, University of Cambridge, Cambridge, UK,Biotechnology Center, Technische Universität Dresden, Dresden, Germany,Max Planck Institute for the Physics of Complex Systems, Dresden, Germany,Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany,Biotechnology Center, Technische Universität Dresden, Dresden, Germany,Cavendish Laboratory, University of Cambridge, Cambridge, UK.Real-time deformability cytometry: on-the-fly cell mechanical phenotyping.[J].Nature methods,2015.
Adamo Andrea,Sharei Armon,Adamo Luigi,Lee ByungKun,Mao Shirley,Jensen Klavs F.Microfluidics-based assessment of cell deformability.[J].Analytical chemistry,2012.
Cheung Karen C,Di Berardino Marco,Schade-Kampmann Grit,Hebeisen Monika,Pierzchalski Arkadiusz,Bocsi Jozsef,Mittag Anja,Tárnok Attila.Microfluidic impedance-based flow cytometry.[J].Cytometry. Part A : the journal of the International Society for Analytical Cytology,2010.
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