1. 武汉科技大学公共卫生学院职业危害识别与控制湖北省重点实验室
2. 上海健康医学院附属周浦医院
纸质出版:2025
移动端阅览
彭晨曦, 龚国傲, 蔡珂, 等. 纳米金复合碳材料修饰的免疫传感器检测血清中CYFRA21-1[J]. 传感器与微系统, 2025,(1):114-118.
彭晨曦, 龚国傲, 蔡珂, 等. 纳米金复合碳材料修饰的免疫传感器检测血清中CYFRA21-1[J]. 传感器与微系统, 2025,(1):114-118. DOI: 10.13873/J.1000-9787(2025)01-0114-05.
构建一种基于羧基化多壁碳纳米管/氮掺杂还原氧化石墨烯/金纳米颗粒(CMWCNTs/N-rGO/AuNPs)复合纳米材料修饰的免疫传感器,用于定量检测人血清中肺癌标志物细胞角蛋白19的可溶性片段(CYFRA21—1)。以尿素作为氮源制备N-rGO
通过超声混合将CMWCNTs、AuNPs与N-rGO结合在一起形成复合材料CMWCNTs/N-rGO/AuNPs。由复合材料修饰的电化学免疫传感器,不仅可以放大电信号,还能通过Au-S键与抗体结合,因此可实现对CYFRA21—1的定量检测。该免疫传感器对人血清中CYFRA21—1的检测在0.1~500 ng/mL的范围内具有良好的线性关系,检出限为0.025 ng/mL。人血清样本的加标回收率为91.75%~109.2%
相对标准偏差小于3.29%(n=6)。该电化学免疫传感器具有良好的特异性、较宽的线性范围和较低的检出限,适合于检测人血清中的CYFRA21—1
具有广阔的应用前景。
Huang Hong,Yang Yongfeng,Zhu Yihan,Chen Hongyu,Yang Ying,Zhang Li,Li Weimin.Blood protein biomarkers in lung cancer[J].Cancer Letters,2022.
Zhao Tongwei,Mao Guangyun,Chen Ming.The Role of Change Rates of CYFRA21-1 and CEA in Predicting Chemotherapy Efficacy for Non-Small-Cell Lung Cancer[J].Computational and Mathematical Methods in Medicine,2021.
Sung Hyuna,Ferlay Jacques,Siegel Rebecca L.,Laversanne Mathieu,Soerjomataram Isabelle,Jemal Ahmedin,Bray Freddie.Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries[J].CA: A Cancer Journal for Clinicians,2021.
Wang Zihua,Yang Shengnan,Wang Yunyun,Feng Wei,Li Binshuai,Jiao Jun,Han Bingkai,Chen Qiang.A novel oriented immunosensor based on AuNPs-thionine-CMWCNTs and staphylococcal protein A for interleukin-6 analysis in complicated biological samples[J].Analytica Chimica Acta,2020.
Parnaz Assari,Amir Abbas Rafati,Azizallah Feizollahi,Roghayeh Asadpour Joghani.Fabrication of a sensitive label free electrochemical immunosensor for detection of prostate specific antigen using functionalized multi-walled carbon nanotubes/polyaniline/AuNPs[J].Materials Science & Engineering C,2020.
Core Laboratory of Translational Medicine, Chinese PLA General Hospital, Beijing, China,National Research Institute for Family Planning, Beijing, China,Core Laboratory of Translational Medicine, Chinese PLA General Hospital, Beijing, China,School of Economics and Management, Beijing Jiaotong University, Beijing, China,School of Economics and Management, Beijing Jiaotong University, Beijing, China,Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education of China), Department of Interventional Therapy, Peking University Cancer Hospital and Institute, Beijing, China.CYFRA21-1 tests in the diagnosis of non-small cell lung cancer: A meta-analysis[J].The International Journal of Biological Markers,2019.
Fen-Ying Kong,Lei Yao,Rong-Fang Li,Heng-Ye Li,Zhong-Xia Wang,Wei-Xin Lv,Wei Wang.Synthesis of nitrogen-doped reduced graphene oxide loading with Au-Ag bimetallic nanoparticles for electrochemical detection of daunorubicin[J].Journal of Alloys and Compounds,2019.
Translational Research Unit, Thoraxklinik at University Hospital Heidelberg, Germany,Translational Lung Research Center (TLRC) Heidelberg, Member of the German Center for Lung Research (DZL), Germany. Electronic address: thomas.muley@med.uni-heidelberg.de.,Roche Diagnostics GmbH, Penzberg, Germany. Electronic address: ying.he@roche.com.,Roche Diagnostics GmbH, Penzberg, Germany. Electronic address: vinzent.rolny@roche.com.,Roche Diagnostics GmbH, Penzberg, Germany. Electronic address: birgit.wehnl@roche.com.,Roche Diagnostics International Ltd, Rotkreuz, Switzerland. Electronic address: achim.escherich@roche.com.,Translational Lung Research Center (TLRC) Heidelberg, Member of the German Center for Lung Research (DZL), Germany,Pathological Institute, University of Heidelberg, Heidelberg, Germany. Electronic address: arne.warth@med.uni-heidelberg.de.,Translational Research Unit, Thoraxklinik at University Hospital Heidelberg, Germany. Electronic address: Christa.stolp@med.uni-heidelberg.de.,Translational Research Unit, Thoraxklinik at University Hospital Heidelberg, Germany,Translational Lung Research Center (TLRC) Heidelberg, Member of the German Center for Lung Research (DZL), Germany. Electronic address: Marc.schneider@med.uni-heidelberg.de.,Translational Research Unit, Thoraxklinik at University Hospital Heidelberg, Germany,Translational Lung Research Center (TLRC) Heidelberg, Member of the German Center for Lung Research (DZL), Germany. Electronic address: Michael.Meister@med.uni-heidelberg.de.,Translational Lung Research Center (TLRC) Heidelberg, Member of the German Center for Lung Research (DZL), Germany,Department of Pneumology and Critical Care Medicine, Thoraxklinik at University Hospital Heidelberg, Germany. Electronic address: fdayyani@uci.edu.,Roche Diagnostics International Ltd, Rotkreuz, Switzerland. Electronic address: felix.herth@med.uni-heidelberg.de..Potential for the blood-based biomarkers cytokeratin 19 fragment (CYFRA 21-1) and human epididymal protein 4 (HE4) to detect recurrence during monitoring after surgical resection of adenocarcinoma of the lung[J].Lung Cancer,2019.
Yan Zeng,Jing Bao,Yanan Zhao,Danqun Huo,Mei Chen,Yanli Qi,Mei Yang,Huanbao Fa,Changjun Hou.A sandwich-type electrochemical immunoassay for ultrasensitive detection of non-small cell lung cancer biomarker CYFRA21-1[J].Bioelectrochemistry,2018.
Farka Zdeněk,Juřík Tomáš,Kovář David,Trnková Libuše,Skládal Petr.Nanoparticle-Based Immunochemical Biosensors and Assays: Recent Advances and Challenges.[J].Chemical reviews,2017.
Jessica K Brinkmeyer,Donald C Moore.Necitumumab for the treatment of squamous cell non-small cell lung cancer:[J].Journal of Oncology Pharmacy Practice,2016.
Joanna Borowiec,Rui Wang,Lihua Zhu,Jingdong Zhang.Synthesis of nitrogen-doped graphene nanosheets decorated with gold nanoparticles as an improved sensor for electrochemical determination of chloramphenicol[J].Electrochimica Acta,2013.
M. Bahgat,A.A. Farghali,W.M.A. El Rouby,M.H. Khedr.Synthesis and modification of multi-walled carbon nano-tubes (MWCNTs) for water treatment applications[J].Journal of Analytical and Applied Pyrolysis,2011.
王登岭,崔传金,陈宏硕,张文思,王义涛,孙淼.基于纳米材料信号放大技术的生物传感器研究进展[J].传感器与微系统,2024(05).
李静,崔传金,龚瑞昆,王春皓,邵斌.免疫传感器芯片表面的抗体固定化方法[J].传感器与微系统,2021(12).
杨倩雯,刘晓风,曾海娟,王金斌.基于纳米材料的电化学免疫传感器及其在蛋白质检测中的研究进展[J].食品科学,2021(21).
王彩纹,邱英梅,邓群,熊国亮.CYFRA21-1三种检测方式在区分肺炎和肺部肿瘤方面的比较[J].江西医药,2019(03).
王淑娴,曲梁静,刁菁,王晓璐,叶海斌,魏鉴腾.生物免疫传感器检测迟缓爱德华氏菌研究[J].传感器与微系统,2017(06).
刘长飞,雷威,刘建伟,赵志国,黄冬梅.CEA、CYFRA21-1、ProGRP血清学检测在肺癌诊断中的应用价值[J].中国美容医学,2012(18).
郭华北,蒋秉坤.血清CYFRA21-1免疫放射分析在非小细胞肺癌诊断中的临床价值[J].中国肿瘤临床,1996(04).
0
浏览量
0
下载量
0
CSCD
关联资源
相关文章
相关作者
相关机构
京公网安备11010802024621