基于酶功能化量子点的人尿液胰蛋白酶传感器

雷少辉; 郑冬云; 刘超; 刘晓军; 朱春楠

doi:10.13873/J.1000-9787(2024)12-0100-05

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基于酶功能化量子点的人尿液胰蛋白酶传感器

设计与制造 | 更新时间：2025-09-11

- 基于酶功能化量子点的人尿液胰蛋白酶传感器
- 暂无标题
- 传感器与微系统 2024年第12期页码：100-104
- 作者机构：
  
  1. 中南民族大学生物医学工程学院
  2. 医学信息分析及肿瘤诊疗湖北省重点实验室
  3. 认知科学实验室(国家民委重点实验室)
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目(21804146,22004134);湖北省自然科学基金资助项目(2023AFB799);中央高校基本科研业务费专项资金项目(CPT22022);横向项目(HZY22110)
- DOI：10.13873/J.1000-9787(2024)12-0100-05
  中图分类号： TP212;R446.12
- 纸质出版：2024
- 稿件说明：
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雷少辉, 郑冬云, 刘超, 等. 基于酶功能化量子点的人尿液胰蛋白酶传感器[J]. 传感器与微系统, 2024,(12):100-104.
雷少辉, 郑冬云, 刘超, 等. 基于酶功能化量子点的人尿液胰蛋白酶传感器[J]. 传感器与微系统, 2024,(12):100-104. DOI： 10.13873/J.1000-9787(2024)12-0100-05.

DOI：

摘要

以葡萄糖氧化酶（GOD）为模板，制备了GOD功能化的锰掺杂硫化锌（GOD-Mn-ZnS）量子点（QDs）

并通过正交实验对QDs的合成条件进行优化。构建了一种检测胰蛋白酶的磷光传感体系，实现了对人体尿液中胰蛋白酶的定量检测。实验结果表明，QDs的磷光猝灭程度与胰蛋白酶的浓度呈良好的线性关系，线性范围为5～100 mg/L

=0.998

检出限为2.21 mg/L。采用标准加入法对健康志愿者的尿液进行检测，加标回收率为97.45%～103.00%。该方法可用于急性胰腺炎患者尿液中胰蛋白酶的快速检测，具有良好的生物医学应用前景。

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