1. 中国科学技术大学微电子学院
2. 中国科学技术大学附属第一医院(安徽省立医院)生殖中心
3. 中国科学技术大学微纳研究与制造中心
纸质出版:2024
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李金坤, 童先宏, 江小华, 等. 基于硅基过滤片的精子优选芯片设计与优化[J]. 传感器与微系统, 2024,(2):81-84+88.
李金坤, 童先宏, 江小华, 等. 基于硅基过滤片的精子优选芯片设计与优化[J]. 传感器与微系统, 2024,(2):81-84+88. DOI: 10.13873/J.1000-9787(2024)02-0081-04.
利用半导体工艺技术构建了一种基于硅基过滤片的精子优选微流控芯片,具有高活力的精子会克服重力,在微孔的引导下游向精子收集池。分别在7
15
30
60 min对收集池中的精子取样,采用计算机辅助精子分析(CASA)系统进行精液常规检测、精子形态学和DNA碎片分析;对硅基过滤片的孔间距和筛选时间进行了优化。结果表明:6μm孔间距和15 min筛选时间为最佳的精子优选方案。所构建的基于硅基过滤片的精子优选芯片,可以有效优选出具有高活力且DNA完整性和形态正常率较高的精子。芯片精液吞吐量大,活动精子回收率较高,有一定的临床价值,同时提供了一种简单易操作的标准化精子优选流程。
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Haotian Wu,Alexandra M. Huffman,Brian W. Whitcomb,Srinihaari Josyula,Suzanne Labrie,Ellen Tougias,Tayyab Rahil,Cynthia K. Sites,J Richard Pilsner.Sperm mitochondrial DNA measures and semen parameters among men undergoing fertility treatment[J].Reproductive BioMedicine Online,2018.
Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco School of Medicine, 550 16th Street, 7th Floor, San Francisco, CA, USA.,Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco School of Medicine, 550 16th Street, 7th Floor, San Francisco, CA, USA.,Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco School of Medicine, 550 16th Street, 7th Floor, San Francisco, CA, USA.,Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco School of Medicine, 550 16th Street, 7th Floor, San Francisco, CA, USA.,Stanford Canary Center for Early Cancer Detection, Stanford University, 3155 Porter Dr, Palo Alto, CA, USA.,Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco School of Medicine, 550 16th Street, 7th Floor, San Francisco, CA, USA.,Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco School of Medicine, 550 16th Street, 7th Floor, San Francisco, CA, USA..Microfluidic sorting selects sperm for clinical use with reduced DNA damage compared to density gradient centrifugation with swim-up in split semen samples.[J].Human reproduction (Oxford, England),2018.
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Nosrati Reza,Vollmer Marion,Eamer Lise,San Gabriel Maria C,Zeidan Krista,Zini Armand,Sinton David.Rapid selection of sperm with high DNA integrity.[J].Lab on a chip,2014.
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