成都医学院公共卫生学院
纸质出版:2024
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苏欣, 朱彤, 许晓寒思, 等. 基于外泌体的适配体传感器的构建与应用[J]. 传感器与微系统, 2024,(1):1-6.
苏欣, 朱彤, 许晓寒思, 等. 基于外泌体的适配体传感器的构建与应用[J]. 传感器与微系统, 2024,(1):1-6. DOI: 10.13873/J.1000-9787(2024)01-0001-06.
基于外泌体的适配体传感器的检测方法具有快速、灵敏、高通量、无创或微创、液体活组织检查和经济高效的分析等诸多优势,成为近年研究热点。综述了目前适配体在检测外泌体、外泌体靶向递送领域的研究进展。首先,介绍了外泌体、适配体和适配体传感器,以及近年常用的适配体筛选技术;其次,阐述基于外泌体的适配体传感器的构建及原理、适配体在外泌体领域的应用;最后,针对目前适配体在外泌体应用领域提出了所遇到的问题和挑战。
Song Zhenguo,Mao Jun,Barrero Roberto A,Wang Peng,Zhang Fengqiu,Wang Tao.Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation.[J].Molecules (Basel, Switzerland),2020.
Tran Phuong H L,Xiang Dongxi,Tran Thao T D,Yin Wang,Zhang Yumei,Kong Lingxue,Chen Kuisheng,Sun Miaomiao,Li Yong,Hou Yingchun,Zhu Yimin,Duan Wei.Exosomes and Nanoengineering: A Match Made for Precision Therapeutics.[J].Advanced materials (Deerfield Beach, Fla.),2020.
Dandan Fang,Dandan Zhao,Shupei Zhang,Yitian Huang,Hong Dai,Yanyu Lin.Black phosphorus quantum dots functionalized MXenes as the enhanced dual-mode probe for exosomes sensing[J].Sensors and Actuators: B. Chemical,2020.
Cui-Fang Ning,Luying Wang,Ya-Fei Tian,Bin-Cheng Yin,Bang-Ce Ye.Multiple and sensitive SERS detection of cancer-related exosomes based on goldâÂÂsilver bimetallic nanotrepangs[J].Analyst,2020.
Key Laboratory of Thin Film and Microfabrication (Ministry of Education), Department of Micro-Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Dongchuan Road 800, Shanghai, 200240, China,Department of Dermatology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, 210008, China,Center of Craniofacial Orthodontics, Department of Oral and Craniomaxillofacial Surgery, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, Shanghai, 200011, China,Key Laboratory of Thin Film and Microfabrication (Ministry of Education), Department of Micro-Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Dongchuan Road 800, Shanghai, 200240, China,Department of Electrical & Computer Engineering, National University of Singapore, 117583, Singapore,Nano-Bionics Division and i-LAB, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China,Key Laboratory of Thin Film and Microfabrication (Ministry of Education), Department of Micro-Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Dongchuan Road 800, Shanghai, 200240, China.RETRACTED: 2D magnetic MoS 2 –Fe 3 O 4 hybrid nanostructures for ultrasensitive exosome detection in GMR sensor[J].Biosensors and Bioelectronics,2020.
Research Center for Analytical Sciences , Northeastern University , Shenyang 110819 , P. R. China.,Research Center for Analytical Sciences , Northeastern University , Shenyang 110819 , P. R. China.,Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology, Ministry of Education , China Medical University , Shenyang 110122 , P. R. China.,Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology, Ministry of Education , China Medical University , Shenyang 110122 , P. R. China.,Research Center for Analytical Sciences , Northeastern University , Shenyang 110819 , P. R. China.,Department of Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology, Ministry of Education , China Medical University , Shenyang 110122 , P. R. China.,Research Center for Analytical Sciences , Northeastern University , Shenyang 110819 , P. R. China..Sensitive Multicolor Visual Detection of Exosomes via Dual Signal Amplification Strategy of Enzyme-Catalyzed Metallization of Au Nanorods and Hybridization Chain Reaction.[J].ACS sensors,2019.
College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China. lifeng@qau.edu.cn.,College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China. lifeng@qau.edu.cn.,College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China. lifeng@qau.edu.cn.,College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China. lifeng@qau.edu.cn.,College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China. lifeng@qau.edu.cn..Aptamer recognition-trigged label-free homogeneous electrochemical strategy for an ultrasensitive cancer-derived exosome assay.[J].Chemical communications (Cambridge, England),2019.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering , China Agricultural University , Beijing 100083 , People's Republic of China.,School of Mechanical and Materials Engineering , Washington State University , Pullman , Washington 99164 , United States.,School of Mechanical and Materials Engineering , Washington State University , Pullman , Washington 99164 , United States.,School of Mechanical and Materials Engineering , Washington State University , Pullman , Washington 99164 , United States.,School of Mechanical and Materials Engineering , Washington State University , Pullman , Washington 99164 , United States.,School of Mechanical and Materials Engineering , Washington State University , Pullman , Washington 99164 , United States.,Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering , China Agricultural University , Beijing 100083 , People's Republic of China.,Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering , China Agricultural University , Beijing 100083 , People's Republic of China.,School of Mechanical and Materials Engineering , Washington State University , Pullman , Washington 99164 , United States..Au@Pd Nanopopcorn and Aptamer Nanoflower Assisted Lateral Flow Strip for Thermal Detection of Exosomes.[J].Analytical chemistry,2019.
Yonghao Wang,Dengwang Luo,Yao Fang,Wanhao Wu,Yongjing Wang,Yaokun Xia,Fang Wu,Chunyan Li,Jianming Lan,Jinghua Chen.An aptasensor based on upconversion nanoparticles as LRET donors for the detection of exosomes[J].Sensors and Actuators: B. Chemical,2019.
Yu An,Tongyu Jin,Yuyuan Zhu,Fan Zhang,Pingang He.An ultrasensitive electrochemical aptasensor for the determination of tumor exosomes based on click chemistry[J].Biosensors and Bioelectronics,2019.
Yu Xiaocheng,He Lei,Pentok Myima,Yang Haowen,Yang Yale,Li Zhiyang,He Nongyue,Deng Yan,Li Song,Liu Tonghua,Chen Xiangyu,Luo Huiwen.An aptamer-based new method for competitive fluorescence detection of exosomes.[J].Nanoscale,2019.
Laura M. Doyle,Michael Zhuo Wang.Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis[J].Cells,2019.
D. Michiel Pegtel,Stephen J. Gould.Exosomes[J].Annual Review of Biochemistry,2019.
Qing Wang,Liyuan Zou,Xiaohai Yang,Xiaofeng Liu,Wenyan Nie,Yan Zheng,Quan Cheng,Kemin Wang.Direct quantification of cancerous exosomes via surface plasmon resonance with dual gold nanoparticle-assisted signal amplification[J].Biosensors and Bioelectronics,2019.
CAS Key Laboratory of Molecular Nanostructure and Nanotechnology, Beijing National Research Center for Molecular Sciences, CAS Research/Education Center for Excellence in Molecule Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. xfang@iccas.ac.cn zhangzhen@iccas.ac.cn.,Department of Medical Oncology, Peking University International Hospital, Beijing 102206, P. R. China.,Echo Biotech Co. Ltd, Beijing 102206, P. R. China.,CAS Key Laboratory of Molecular Nanostructure and Nanotechnology, Beijing National Research Center for Molecular Sciences, CAS Research/Education Center for Excellence in Molecule Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. xfang@iccas.ac.cn zhangzhen@iccas.ac.cn.,CAS Key Laboratory of Molecular Nanostructure and Nanotechnology, Beijing National Research Center for Molecular Sciences, CAS Research/Education Center for Excellence in Molecule Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. xfang@iccas.ac.cn zhangzhen@iccas.ac.cn and University of Chinese Academy of Sciences, Beijing 100049, P. R. China.,CAS Key Laboratory of Molecular Nanostructure and Nanotechnology, Beijing National Research Center for Molecular Sciences, CAS Research/Education Center for Excellence in Molecule Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. xfang@iccas.ac.cn zhangzhen@iccas.ac.cn and University of Chinese Academy of Sciences, Beijing 100049, P. R. China.,Echo Biotech Co. Ltd, Beijing 102206, P. R. China.,Echo Biotech Co. Ltd, Beijing 102206, P. R. China.,CAS Key Laboratory of Molecular Nanostructure and Nanotechnology, Beijing National Research Center for Molecular Sciences, CAS Research/Education Center for Excellence in Molecule Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. xfang@iccas.ac.cn zhangzhen@iccas.ac.cn and University of Chinese Academy of Sciences, Beijing 100049, P. R. China..Aptamer-based fluorescence polarization assay for separation-free exosome quantification.[J].Nanoscale,2019.
Huang Rongrong,He Lei,Xia Yanyan,Xu Hongpan,Liu Chang,Xie Hui,Wang Su,Peng Lijun,Liu Yufeng,Liu Yuan,He Nongyue,Li Zhiyang.A Sensitive Aptasensor Based on a Hemin/G-Quadruplex-Assisted Signal Amplification Strategy for Electrochemical Detection of Gastric Cancer Exosomes.[J].Small (Weinheim an der Bergstrasse, Germany),2019.
Lyu Yan,Cui Dong,Huang Jiaguo,Fan Wenxuan,Miao Yansong,Pu Kanyi.Near-Infrared Afterglow Semiconducting Nano-Polycomplexes for the Multiplex Differentiation of Cancer Exosomes.[J].Angewandte Chemie (International ed. in English),2019.
Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences , Aptamer Engineering Center of Hunan Province, Hunan University , Changsha , Hunan 410082 , China.,Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences , Aptamer Engineering Center of Hunan Province, Hunan University , Changsha , Hunan 410082 , China.,Hunan Provincial Key Laboratory of Forestry Biotechnology, College of Life Science and Technology , Central South University of Forestry & Technology , Changsha 410004 , China.,Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences , Aptamer Engineering Center of Hunan Province, Hunan University , Changsha , Hunan 410082 , China.,Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences , Aptamer Engineering Center of Hunan Province, Hunan University , Changsha , Hunan 410082 , China.,Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences , Aptamer Engineering Center of Hunan Province, Hunan University , Changsha , Hunan 410082 , China.,Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences , Aptamer Engineering Center of Hunan Province, Hunan University , Changsha , Hunan 410082 , China.,Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences , Aptamer Engineering Center of Hunan Province, Hunan University , Changsha , Hunan 410082 , China.,Center for Research at Bio/Nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics , University Health Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida , Gainesville , Florida 32611-7200 , United States..Aptamer-Functionalized Exosomes: Elucidating the Cellular Uptake Mechanism and the Potential for Cancer-Targeted Chemotherapy.[J].Analytical chemistry,2019.
Huang Lin,Wang Dian-Bing,Singh Netrapal,Yang Fang,Gu Ning,Zhang Xian-En.A dual-signal amplification platform for sensitive fluorescence biosensing of leukemia-derived exosomes.[J].Nanoscale,2018.
Zhang Qiuxia,Wang Feng,Zhang Huixin,Zhang Youyu,Liu Meiling,Liu Yang.Universal Ti3C2 MXenes Based Self-Standard Ratiometric Fluorescence Resonance Energy Transfer Platform for Highly Sensitive Detection of Exosomes.[J].Analytical chemistry,2018.
Dong Huilei,Chen Hongfei,Jiang Juqian,Zhang Hui,Cai Chenxin,Shen Qingming.Highly Sensitive Electrochemical Detection of Tumor Exosomes Based on Aptamer Recognition-Induced Multi-DNA Release and Cyclic Enzymatic Amplification.[J].Analytical chemistry,2018.
Wang Yu-Min,Liu Jin-Wen,Adkins Gary Brent,Shen Wen,Trinh Michael Patrick,Duan Lu-Ying,Jiang Jian-Hui,Zhong Wenwan.Enhancement of the Intrinsic Peroxidase-Like Activity of Graphitic Carbon Nitride Nanosheets by ssDNAs and Its Application for Detection of Exosomes.[J].Analytical chemistry,2017.
Jiang Ying,Shi Muling,Liu Yuan,Wan Shuo,Cui Cheng,Zhang Liqin,Tan Weihong.Aptamer/AuNP Biosensor for Colorimetric Profiling of Exosomal Proteins.[J].Angewandte Chemie (International ed. in English),2017.
Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology , Beijing 100029, China.,Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.,Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha 410082, China.,Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.,Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.,Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.,Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.,Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha 410082, China.,Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.,Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.,Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.,Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.,Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha 410082, China.,Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.,Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha 410082, China.,Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States.,Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology , Beijing 100029, China.,Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University , Changsha 410082, China.,Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, McKnight Brain Institute, UF Genetics Institute, University of Florida , Gainesville, Florida 32611-7200, United States..Aptasensor with Expanded Nucleotide Using DNA Nanotetrahedra for Electrochemical Detection of Cancerous Exosomes.[J].ACS nano,2017.
Jia Wenting,Ren Caiping,Wang Lei,Zhu Bin,Jia Wei,Gao Menghui,Zeng Fei,Zeng Liang,Xia Xiaomeng,Zhang Xiaobing,Fu Ting,Li Shasha,Du Can,Jiang Xingjun,Chen Yuxiang,Tan Weihong,Zhao Zilong,Liu Weidong.CD109 is identified as a potential nasopharyngeal carcinoma biomarker using aptamer selected by cell-SELEX.[J].Oncotarget,2016.
Nguyen Van-Thuan,Kwon Young Seop,Kim Jae Hoon,Gu Man Bock.Multiple GO-SELEX for efficient screening of flexible aptamers.[J].Chemical communications (Cambridge, England),2014.
Park Jee-Woong,Tatavarty Rameshwar,Kim Dae Woo,Jung Hee-Tae,Gu Man Bock.Immobilization-free screening of aptamers assisted by graphene oxide.[J].Chemical communications (Cambridge, England),2012.
Regina Stoltenburg,Nadia Nikolaus,Beate Strehlitz.Capture-SELEX: Selection of DNA Aptamers for Aminoglycoside Antibiotics[J].Journal of Analytical Methods in Chemistry,2012.
Choon Bok Joeng,Javed H. Niazi,Su Jin Lee,Man Bock Gu.ssDNA aptamers that recognize diclofenac and 2-anilinophenylacetic acid[J].Bioorganic & Medicinal Chemistry,2009.
Cruz-Aguado Jorge A,Penner Gregory.Determination of ochratoxin a with a DNA aptamer.[J].Journal of agricultural and food chemistry,2008.
Mosing Renee K,Mendonsa Shaun D,Bowser Michael T.Capillary electrophoresis-SELEX selection of aptamers with affinity for HIV-1 reverse transcriptase.[J].Analytical chemistry,2005.
Stoltenburg R,Reinemann C,Strehlitz B.FluMag-SELEX as an advantageous method for DNA aptamer selection.[J].Analytical and bioanalytical chemistry,2005.
Daniels Dion A,Chen Hang,Hicke Brian J,Swiderek Kristine M,Gold Larry.A tenascin-C aptamer identified by tumor cell SELEX: systematic evolution of ligands by exponential enrichment.[J].Proceedings of the National Academy of Sciences of the United States of America,2003.
Bruno J G.In VitroSelection of DNA to Chloroaromatics Using Magnetic Microbead-Based Affinity Separation and Fluorescence Detection[J].Biochemical and biophysical research communications,1997(1).
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