南昌大学第一附属医院消化内科江西省消化疾病研究所
纸质出版:2023
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张洪丽, 王婷, 张焜和. 核酸适配子的结构特征及其对结合性能的影响[J]. 传感器与微系统, 2023,(8):1-5+10.
张洪丽, 王婷, 张焜和. 核酸适配子的结构特征及其对结合性能的影响[J]. 传感器与微系统, 2023,(8):1-5+10. DOI: 10.13873/J.1000-9787(2023)08-0001-05.
核酸适配子是从人工合成的随机单链寡核苷酸文库中筛选出的生物分子特异性配体,其与靶分子的结合性能类似于抗体,但具有热稳定性、制备方便、易于进行结构修饰等优点,因而广泛应用于生物医药领域的分子检测。核酸适配子的结构直接影响其与靶分子的结合性能,包括一级结构、二级结构、三级结构,以及G—四联体等特殊结构。核酸适配子的高级结构不但受其自身结构特点的影响,也受其周围环境理化因素的影响。深入了解核酸适配子结构特点及其对结合靶标影响,进而进行针对性结构修饰及截短,可获得更优性能的核酸适配子,促进其实际应用,并为分析其作用机制提供依据。基于核酸适配子结构特点而设计的适配子传感器,可获得良好的靶标检测性能。
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