甘肃省科学院传感技术研究所甘肃省传感器与传感技术重点实验室
纸质出版:2023
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李云霞, 马莉萍, 马生龙, 等. 基于氧化石墨烯的适配体生物传感器检测Hg2+[J]. 传感器与微系统, 2023,(3):31-35.
李云霞, 马莉萍, 马生龙, 等. 基于氧化石墨烯的适配体生物传感器检测Hg2+[J]. 传感器与微系统, 2023,(3):31-35. DOI: 10.13873/J.1000-9787(2023)03-0031-05.
针对汞离子(Hg
2+
)作为一种毒性强且污染较为普遍的重金属离子,会严重危害人体健康的问题,设计了Hg
2+
特异识别探针T
28
-FAM
T
28
-FAM可与Hg
2+
共价结合形成稳定的T-Hg
2+
-T配位结构,以T
28
-FAM为荧光信号源,氧化石墨烯(GO)为猝灭剂,构建基于T
28
-FAM/GO的适配体荧光传感器,实现对Hg
2+
浓度的高灵敏定量检测。该传感器检测Hg
2+
的线性范围为100~600 nmol/L
相关系数R
2
为0.996 12
检出限为0.458 nmol/L。9种干扰离子(Co
2+
Cu
2+
Cl
-
Mg
2+
Ca
2+
Ag
+
Fe
2+
Pb
2+
NO
-
3
)的存在不影响该方法检测Hg
2+
表明该方法对Hg
2+
检测具有较好的特异性。在自来水样Hg
2+
加标检测实验中,平均回收率为95.77%
有望用于实际样品的检测。
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