Immunosensor is widely used in the fields of environmental detection and biomedicine because it has advantages of fast response

sensitive and low cost.Antibody is the core reagent of immunology detection.Fixing antibody on the surface of sensor efficiently is the key to obtain high sensitivity detection.Progresses in the fields of material chemistry and crosslinker chemistry promote rapid development of antibody immobilization techniques.The characteristics of various antibody immobilization methods on the sensor surface

including physical adsorption

covalent binding by carboxyl group

amino group

glycosyl group and the fixation method of biotin-affidin affinity

are emphatically reviewed

and the development direction of methods of antibody immobilization are prospected.