In practical application

the standard concentration of AFB1 is very low.In order to increase the capture ability and detection sensitivity of sensors

electrochemical and fluorescence probes are prepared by modifying sucrase and carbon quantum dots on aptamers

respectively

and electrochemical and fluorescence aptasensors based on the sandwich structure of antibody—AFB1—detection probe are constructed.AFB1 samples with different concentrations are also detected.The results show that the linear detection range of electrochemical aptasensor combined with the glucose meter are 0.5～5 ng/mL.Linear detection range of fluorescence aptasensor are 0.5～3 ng/mL.The sensitivity and specificity of the two sensors are compared.And it is found that the specificity is similar but the sensitivity is two orders of magnitude different.Combining the detection instruments of the two sensors

it can be seen that the former is convenient to carry and has a wide measurement range

which is suitable for the rough measurement in the actual environment combined with the standard solution.However

fluorescence aptasensor has high sensitivity

but the detection equipment is expensive and inconvenient to carry

which is more suitable for accurate analysis in laboratory after sampling.