An integrated microfluidic device

"alveolus-on-a-chip" is designed and fabricated based on micro-electro-mechanical system（MEMS） technology and polydimethylsiloxane（PDMS） soft lithography technology.The device is suitable for cell culture on a chip and trace volatile metabolites capture under a three-dimensional environment.The capture gas is analyzed by mass spectrometric technique.Experimental results show that the microfluidic chip achieves lung cancer cells culture on the chip and trace metabolic gases capture.Compared with other existing methods

the microfluidic chip shows advantages in simpler structure and less sample consuming

which provides an efficient way to analyze on metabolic gases from cells in vitro.