A micro-fluidic chip with integrated microband array electrode is designed and fabricated for the detection of human cardiac troponin I（ c Tn I） in the early diagnosis of acute myocardial infarction. The chip consists of a polydimethylsiloxane（ PDMS） channel plate and a glass electrode plate. One micro-channel to immobilize c Tn I coating antibody is integrated on the PDMS channel plate. Microband array electrode for the concentration detection of c Tn I is integrated on the glass electrode plate. c Tn I coating antibody is immobilized onto the internal surface of the PDMS channel on which protein G layer is generated by silanization. The detection experiments are performed with the double antibody sandwich method. Cyclic voltammograms are obta

ined by the oxidation peak current proportionally to the concentration of enzymatic product

p-aminophenol（ PAP）. The theoretical limit of detection is 0. 4 ng/m L within 8 min

the linear detection range is 1

1 000 ng/m L.