Analyze on fluorescence excitation characteristics of microcystis flos-aquae chlorophyll a using image processing techniques by fluorescence spectrometer

microcystis flos aquae concentration automatic detection method based on algae fluorescence excitation effect is proposed. Fluorescence 430 nm is used as excitation light source

wavelength 680 nm microcystis flos-aquae fluorescence microscopic image is obtained

remove influence of other incident light by red filter chip

the image contains only microcystis flos-aquae fluorescence characteristics;and grayscale

binarization

filtering

count and algae liquid concentration measurement are carried out on the obtained fluorescence image. Experiments show that this method can distinguish the algae and impurities very well

concentration measurement results is accurate and computation speed is fast and provides a new convenient method for the algae concentration detection.