适体和蛋白质解离常数检测方法比较分析

苏雪; 何逸婷; 林俊生

doi:10.13873/J.1000-9787(2016)11-0047-04

Chinese

您当前的位置：

首页 >

文章列表页 >

适体和蛋白质解离常数检测方法比较分析

研究与探讨 | 更新时间：2025-09-11

- 适体和蛋白质解离常数检测方法比较分析
- Comparative analysis of detection methods for dissociation constant of aptamer and protein
- 传感器与微系统 2016年35卷第11期页码：47-50
- 作者机构：
  
  华侨大学生物医学学院
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目（81270734）;华侨大学高层次人才项目（13Y0391）
- DOI：10.13873/J.1000-9787(2016)11-0047-04
  中图分类号： Q5-33;TP212
- 纸质出版：2016
- 稿件说明：
移动端阅览
[1]苏雪,何逸婷,林俊生.适体和蛋白质解离常数检测方法比较分析[J].传感器与微系统,2016,35(11):47-50.

SU Xue, HE Yi-ting, LIN Jun-sheng. Comparative analysis of detection methods for dissociation constant of aptamer and protein[J]. 2016, 35(11): 47-50.
[1]苏雪,何逸婷,林俊生.适体和蛋白质解离常数检测方法比较分析[J].传感器与微系统,2016,35(11):47-50. DOI： 10.13873/J.1000-9787(2016)11-0047-04.

SU Xue, HE Yi-ting, LIN Jun-sheng. Comparative analysis of detection methods for dissociation constant of aptamer and protein[J]. 2016, 35(11): 47-50. DOI： 10.13873/J.1000-9787(2016)11-0047-04.

摘要

对基于石英晶体微天平（QCM）生物传感器的两种检测核酸适体与蛋白质解离常数方法进行了比较

提出来一种更加精确合理的检测流程。以凝血酶和凝血酶适体TBA15为模型

耗散型石英晶体微天平（QCM-D）为传感器

实时检测末端修饰巯基适体的固定、表面封闭剂对非特异性结合位点封闭以及两种不同的蛋白进样方式引起的频率响应

实验数据拟合得到解离常数。不同蛋白的进样方式得到的解离常数不同

非特异性位点的封闭也同样影响解离常数的检测。从低浓度到高浓度依次通入固定体积蛋白的进样方式

实验重复性高且消耗样品量小于1μg

是较为理想的检测方式。

Abstract

Compare the two detection methods of dissociated constant of aptamer and protein based on quartz crystal microbalance（ QCM） biosensor

a protocol with high accuracy and reasonability is introduced. Thrombin and thrombin aptamer named TBA15 are used as models. The immobilization of thiolated aptamer

the block of the non-specific site and the signal response induced by two different sampling methods are monitored by QCM with dissipation（ QCM-D） biosensor in real time and dissociated constant are calculated by data fitting. Dissociated constant are different when different sampling methods are used

and different block agent applied

blocking of nonspecific site. The sampling method with fixed bulk of protein from low concentration to high concentration are proved ideal method with high repeatability and fewer regents needed which is less than 1 μg.

关键词

Keywords

references

Applications of aptamers for chemistry analysis, medicine and food security [J]. Rongrong Huang,Zhijiang Xi,Nongyue He. Science China（Chemistry） . 2015(07)

石英晶体微天平的研究进展综述 [J]. 陈超杰,蒋海峰. 传感器与微系统 . 2014(05)

ZnO纳米棒修饰的QCM气体传感器检测NH 3 研究 [J]. 张嘉琪,胡馨升,胡发志,齐高璨,袁志好. 传感器与微系统 . 2014(04)

赤芍801对白细胞介素-6与其受体相互作用影响的研究 [J]. 崔莹光,廉文静,黄加栋. 传感器与微系统 . 2011(06)

适体电化学生物传感器研究进展 [J]. 李晓霞,申丽华,漆红兰. 传感器与微系统 . 2007(12)

An enzyme-free surface plasmon resonance biosensor for real-time detecting microRNA based on allosteric effect of mismatched catalytic hairpin assembly [J] . Jianbo Li,Pinhua Lei,Shijia Ding,Ye Zhang,Jianru Yang,Quan Cheng,Yurong Yan. Biosensors and Bioelectronics . 2016

Nanogravimetric and Optical Characterizations of Thrombin Interaction with a Self-Assembled Thiolated Aptamer [J] . Jane Politi,Ilaria Rea,Fabrizia Nici,Principia Dardano,Monica Terracciano,Giorgia Oliviero,Nicola Borbone,Gennaro Piccialli,Luca De Stefano,Maria Luz Rodríguez-Méndez. Journal of Sensors . 2016

Optimisation of an electrochemical impedance spectroscopy aptasensor by exploiting quartz crystal microbalance with dissipation signals [J] . Nello Formisano,Pawan Jolly,Nikhil Bhalla,Mary Cromhout,Shane P. Flanagan,Ronen Fogel,Janice L. Limson,Pedro Estrela. Sensors & Actuators: B. Chemical . 2015

Lysozyme specific aptamer immobilized MCM-41 silicate for single-step purification and quartz crystal microbalance （QCM）-based determination of lysozyme from chicken egg white [J] . Gulay Bayramoglu,V. Cengiz Ozalp,Meltem Yilmaz,Ulku Guler,Bekir Salih,M. Yakup Arica. Microporous and Mesoporous Materials . 2015

Amplified QCM-D biosensor for protein based on aptamer-functionalized gold nanoparticles [J] . Qiang Chen,Wei Tang,Dingzhong Wang,Xiaojie Wu,Na Li,Feng Liu. Biosensors and Bioelectronics . 2010 (2)

Methods for measuring aptamer-protein equilibria: A review [J] . Meng Jing,Michael T. Bowser. Analytica Chimica Acta . 2010 (1)

Aptamer-based piezoelectric quartz crystal microbalance biosensor array for the quantification of IgE [J] . Chunyan Yao,Yongzhi Qi,Yuhui Zhao,Yang Xiang,Qinghai Chen,Weiling Fu. Biosensors and Bioelectronics . 2008 (8)

A QCM-D-based biosensor for E. coli O157:H7 highlighting the relevance of the dissipation slope as a transduction signal [J] . Charles Poitras,Nathalie Tufenkji. Biosensors and Bioelectronics . 2008 (7)

Aptamer-based biosensors [J] . Shiping Song,Lihua Wang,Jiang Li,Chunhai Fan,Jianlong Zhao. Trends in Analytical Chemistry . 2007 (2)

Aptamer-based biosensors for the detection of HIV-1 Tat protein [J] . S. Tombelli,M. Minunni,E. Luzi,M. Mascini. Bioelectrochemistry . 2005 (2)

浏览量

150

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

核酸适体对荧光共振适体传感器灵敏度的影响

蛋白质在硅烷膜表面吸附特性的压电传感监测