重庆医科大学生物医学工程研究室
纸质出版:2015
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[1]张婷婷,张瑞英,陈镇,罗义庭,熊兴良.比色生物传感器检测Cd~(2+)[J].传感器与微系统,2015,34(05):143-145.
ZHANG Ting-ting, ZHANG Rui-ying, CHEN Zhen, et al. Colorimetric biosensor for Cd2+ detection[J]. 2015, 34(5): 143-145.
[1]张婷婷,张瑞英,陈镇,罗义庭,熊兴良.比色生物传感器检测Cd~(2+)[J].传感器与微系统,2015,34(05):143-145. DOI: 10.13873/J.1000-9787(2015)05-0143-03.
ZHANG Ting-ting, ZHANG Rui-ying, CHEN Zhen, et al. Colorimetric biosensor for Cd2+ detection[J]. 2015, 34(5): 143-145. DOI: 10.13873/J.1000-9787(2015)05-0143-03.
基于Cd2+—DNA复合物介导AuNPs聚集构建了比色生物传感器
并用于检测镉离子(Cd2+)。单链DNA(ssDNA)通过Au-N键与AuNPs静电吸附
带负电荷的DNA互相排斥
使DNA-AuNPs能稳定分散在盐溶液中。Cd2+能优先结合DNA
AuNPs暴露在盐溶液中发生聚集
溶液由红色变成蓝紫色。Pb2+
Cu2+有轻微干扰
其它金属离子无干扰
裸眼可观察的检测下限低至0.5μmol/L。该方法成功用于环境水样中Cd2+检测。
A new colorimetric biosensor for the detection of Cd2 +based on Cd2 +-DNA complexes inducing the the aggregation of Au NPs is constructed. Single-stranded DNA(ssDNA) can be absorbed onto Au NP surfaces through Au-N electrostatic attraction. Owing to the mutual repulsion of the negative charge of DNA on each AuNP surface
monodisperse Au NPs existed in the salt solution. Cd2 +can priority in combination with DNA
AuNPs exposed in salt solution
aggregation of the Au NPs occurs and
hence
the color of the solution changes from red to purple blue. Most of metal ions had no obvious interference except for a slight from Pb2 +
Cu2 +. The detection limit of Cd2 +can be as low as 0. 5 μmol / L. The proposed method is successfully applied to determine the concentration of Cd2 +in environmental samples.
纳米金比色法快速检测水中重金属的研究进展 [J]. 戚红卷,陈雯雯,岳丽君,王卓,孟洋,刘雪林. 环境化学 . 2013(01)
检测重金属离子的酶膜生物传感器的构建 [J]. 寇冬梅,张进忠,杨兵,魏世强,张明晓. 环境科学与技术 . 2008(09)
重金属污染物的免疫学检测技术研究进展 [J]. 江天久,牛涛. 生态环境 . 2005(04)
Sensitive and selective colorimetric detection of cadmium(II) using gold nanoparticles modified with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole [J] . Ai-Jun Wang,Han Guo,Ming Zhang,Dan-Ling Zhou,Rui-Zhi Wang,Jiu-Ju Feng. Microchimica Acta . 2013 (11-1)
Chemical and biological properties of toxic metals and use of chelating agents for the pharmacological treatment of metal poisoning [J] . Maria Stefania Sinicropi,Diana Amantea,Anna Caruso,Carmela Saturnino. Archives of Toxicology . 2010 (7)
The electrochemical detection of cadmium using surface-immobilized DNA [J] . Elicia L.S. Wong,Edith Chow,J. Justin Gooding. Electrochemistry Communications . 2006 (4)
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