A glucose sensor based on hollow micro-needle array is developed to pierce skin with slight pain and achieve continuous monitoring on human blood glucose concentration. The micro-needle array is constituted by three same spacing micro-needles

used as working electrode

auxiliary electrode and reference electrode of the biosensor

respectively

in which

working electrode and auxiliary electrode are both made of stainless hollow steel micro-needle with sputtered Ti / Pt films on the surface

use glutaraldehyde-BSA cross-linking method to fix glucose oxidase（ GOD） only on the tip of the micro-needle of working electrode; reference electrode is made of Ag / Ag Cl solid micro-needle. The test results show that the output current of the sensor is linear

in the range of 0

2

1 mmol·L- 1with glucose concentration

and the sensitivity is 0. 575 μA /（ mmol·L- 1）

and the response time is 16 s

and with good anti-jamming performance.