To address the need for long-term monitoring of electrical activity within in vitro neuronal networks

a 60-channel microelectrode array(MEA)system is designed and validated.The array is fabricated on a silicon substrate using microfabrication processes

with silver as the conductive layer and silicon nitride as the insulating layer.The electrode diameter and spacing are 20μm and 40μm

respectively.The system integrates low-noise amplification and multiplexed acquisition modules

enabling simultaneous recording of action potentials and local field potentials.Experimental results based on cultured primary mouse hippocampal neurons demonstrate that the system effectively captures the developmental dynamics of the neuronal network

from initial random firing(mean firing rate 0.12±0.02Hz)to synchronized bursting at maturity(0.71±0.07Hz)

achieving a spatial coverage of 85%.This study provides a high-throughput

non-invasive

multi-channel recording platform for neuroelectrophysiology research.