To address the key technical bottleneck of precise targeted delivery of functional cells

a fabrication method for magnetic microcellular spheroid robots based on cell self-assembly and their automated micromanipulation system are proposed.By co-culturing ferroferric oxide(Fe

3

O

4

) magnetic nanoparticles with cells on a non-adherent surface

the cells are induced to spontaneously form a three-dimensional magnetic cell spheroid structure.This structure not only maintains the functional integrity of the cells but also endows them with excellent magnetoresponsive properties.On this basis

a

permanent magnet-based magnetic control microsystem is constructed

and a navigation strategy without image feedback is developed to realize the precise manipulation of microswarms composed of cell spheroids.Experimental results show that the system can efficiently achieve automated aggregation of magnetic cell spheroids in an in vitro environment and successfully guide them to complete directional migration along a specified path

demonstrating good motion stability.This technology provides a technical platform with stable structure

excellent biocompatibility

and convenient manipulation for precise delivery in cell therapy

and exhibits promising prospects for clinical translation.