重庆医科大学医学检验系
纸质出版:2008
移动端阅览
[1]谢国明,熊兴良,罗鹏,邹超世,周觅.一种基于酶传感器的血酒精便携式检测仪的研究[J].传感器与微系统,2008(08):42-44.
XIE Guo-ming, XIONG Xing-liang, LUO Peng, et al. Study on blood alcohol portable apparatus based on enzyme biosensor[J]. 2008, (8): 42-44.
介绍了一种直接测定末梢血酒精浓度的检验仪器。基于丝网印刷技术制备一次性碳电极
用Nafion固定麦尔多拉蓝(Meldola’sblue
MB)作为电子介体
通过交联法用戊二醛将乙醇脱氢酶(ADH)和氧化型辅酶Ⅰ(NAD+)固定于Nafion-MB修饰的电极上
制成检测ADH催化酒精产生电化学反应形成的电流的末梢血酒精一次性生物传感器。以单片机EM78P458为中央处理控制器
负责检测生物传感器反应输出的电流
并对信号进行放大、处理和显示。实验结果表明:血酒精酶传感器便携式检测仪与自动生化分析仪测得血酒精浓度值的相关性良好。提供了一种准确检测血酒精浓度的新技术和途径。
A new apparatus which detects directly the blood alcohol concentration(BAC)is introduced.This biosensor comprises a screen-printed carbon electrode modified with alcohol dehydrogenase(ADH)
nicotinamide adenine dinucleotide(NAD+) cofactor and Meldola’s blue(MB)adsorbed on nafion film.It is employed for measuring the current which is generated by the electrochemical reaction catalyzed by alcohol dehydrogenase.And a single chip microcomputer
EM78P458
is used as central control unit.It measures the output of current conduced by the biosensor.Then
the detected current is amplified and processed
and the results are displayed on LCD.Experiment results show that the output of apparatus appears good relativity with that from the automatic biochemistry analyzer.A new accurate way for detecting BAC is provided.
血乙醇浓度分析方法及其评价 [J]. 李琼,谢国明,徐华建,阳强. 分析仪器 . 2005(03)
Mediatorless voltammetric oxidation of NADH and sensing of ethanol [J] . C. Retna Raj,S. Behera. Biosensors and Bioelectronics . 2005 (6)
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